Temperature & Taste: Kernel: Neutral in TCM (slightly Warm in the West); Moist. Prev Next List. The expression of mitosis-related protein Cdc25c was impaired upon TSG treatment. The basal concentration of histamine in the supernatant of rat colon tissue was  ng/mL (). Root of Prunus persica serves as another important ingredient in traditional Chinese medicine prescriptions. A muscarinic receptor antagonist, atropine, was used to determine whether the gut-stimulatory effect was mediated through cholinergic activity. In contrast, the addition of DMSO had no effects on cellular migration compared with the control (blank) (Figures 3(a)–3(g)). Location. However, even the first line palliative treatment medicine, sorafenib, contributes modest survival benefit (about 2.5 months) to advanced HCC patient [2, 3]. Copyright © 2015 Wei Han et al. TTX (1 μM) affected spontaneous contractions of isolated rat colon strip but did not prevent the EAE-induced contraction (Figure 2), indicating that enteric neurons are not involved in the EAE-induced contraction. They are frequently used as an ingredient in a variety of Kampo (Chinese medicine) prescriptions, particularly those used to treat women’s diseases. Five populations of archaeological peach stones recovered from Zhejiang Province, China, document peach use and … In summary, EAE was shown to stimulate rat colonic muscle contraction in a dose-dependent manner. (b) Group summary data of EAE-induced changes in the amplitude. In this study, we presented the evidence that ethanol extract of root of Prunus persica can inhibit the growth of HepG2 cells in vitro by impairing cell cycle and migration. It is known that the major product of mast cell is histamine [23]. compressa (Loud.) Comments: The peach fruit tree (Prunus persica) appears to have originated in China, where it was mentioned in literature several centuries before Christ. … Prunus persica var. EAE (10−8–10−5 g/mL) caused a concentration-dependent stimulatory effect in rat colonic tissue. Mast cells have been reported to be associated with gastrointestinal motility in various conditions [20]. Mast cell analysis was then used to confirm the involvement of mast cells in the EAE-treated group. Prunus persica in Traditional Chinese Medicine Background. Light smell and lightly bitter taste. Tack and S. Müller-Lissner, “Treatment of chronic constipation: current pharmacologic approaches and future directions,”, M. H. Mehmood, A. J. Al-Rehaily, R. A. ((a)–(f)) Representative images of wound healing assay of HepG2 cells treated with 0.34 mg/ml TSG or equal volume of DMSO or blank medium at the time point of 0 and 48 hours. Based on their genome‐wide single‐nucleotide polymorphisms merged with published resequencing data of 79 Chinese P. mume cultivars, we inferred complete and ongoing population differentiation in P. mume. Mast cells produce histamine which can stimulate enteric smooth muscle contraction [21, 22]. Prunus persica 9 (Pru p 9) is a new occupational allergen from peach tree pollen involved in rhinitis and asthma. It belongs to … Classifications < > Search. They are frequently used as an ingredient in a variety of Kampo (Chinese medicine) prescriptions, particularly those used to treat women’s diseases. The concentration of the stock ethanol extract of TSG is 0.086 g/ml which almost reaches saturation. Publication Author Yeung. 3G. Prunus persica(L.) BATSCH(Rosaceae) seeds are well known as a traditional medicine (Persicae Semen; Tounin, Taoren in Chinese) in Japan, China, and other Asian coun- tries. Thus, the involvement of histamine in EAE-induced response was investigated by the H1 receptor antagonist pyrilamine and the H2 receptor antagonist cimetidine. The plate was analyzed with BioTek synergy 2 microplate reader (USA). Statistical analyses were performed using one-way analysis of variance followed by Bonferroni correction or Student’s -test. The species name resulted from an early misconception that the peach originated in Persia. All treatments were performed in 4 replicates on the same plate. In this study, we investigated the antitumor role of ethanol extract of root of peach tree (Prunus persica (L.) Batsch and hereafter designated as TSG in short of its Chinese name), which is an important ingredient in Chinese medicine prescription, in liver cancer cell HepG2. Shin, S.-B. Pretreatment with cimetidine (10 μM) or pyrilamine (1 μM) could inhibit the EAE-induced contraction (Figure 2). reported that Prunus persica seed-derived glycosides showed antitumor activity in vitro and in vivo [7]. These data indicate that EAE has significant prokinetic activity and acts by a mechanism that mainly involves mast cell degranulation. It bears edible juicy fruits with various characteristics, most called peaches and others, nectarines. Toluidine blue staining and immunofluorescence showed that, following treatment with EAE, the number of mast cells was increased compared with controls. Although early-staged HCC can be subjected to curative surgical treatments, like tumor resection and ablation, the majority of HCC patients often already progress to intermediate or advanced disease stage upon clinical diagnosis. To begin the anti-HCC activity investigation of root of Prunus persica, we firstly performed the ethanol extraction of root of Prunus persica (TSG thereafter). Cell cycle analysis was conducted with the cell cycle detection kit (KeyGen, China) according to the manufacturer’s instruction. Negative controls were performed by omitting one primary antibody. Order) 2 YRS . TSG inhibited growth of HepG2 cells. Although prokinetics and laxatives are used for gut motility disorders, these become less efficient with long-term treatment [2]. It belongs to the Rosaceae family, Prunoideae subfamily, and Amygdalus subgenus. The authors confirm that they have no conflict of interests. The bulk ethanol extract was vacuum dried and quantified. The cultivated/domesticated peach (Prunus persica var. HepG2 cells were seeded onto 6-well plate at a density of per well. Mast cells were determined in the EAE-treated group and control group. Duan, Y. P. Tang et al., “Taoren-Honghua herb pair and its main components promoting blood circulation through influencing on hemorheology, plasma coagulation and platelet aggregation,”, M. Shirosaki, T. Koyama, and K. Yazawa, “Suppressive effect of peach leaf extract on glucose absorption from the small intestine of mice,”, M. Shirosaki, Y. Goto, S. Hirooka, H. Masuda, T. Koyama, and K. Yazawa, “Peach leaf contains multiflorin A as a potent inhibitor of glucose absorption in the small intestine in mice,”, M. Y. Heo, S. H. Kim, H. E. Yang, S. H. Lee, B. K. Jo, and H. P. Kim, “Protection against ultraviolet B- and C-induced DNA damage and skin carcinogenesis by the flowers of, Y. H. Kim, H. E. Yang, J. H. Kim, M. Y. Heo, and H. P. Kim, “Protection of the flowers of Prunus persica extract from ultraviolet B-induced damage of normal human keratinocytes,”, Najeeb-ur-Rehman, M. H. Mehmood, K. M. Alkharfy, and A.-H. Gilani, “Prokinetic and laxative activities of, H. Eutamene, V. Theodorou, N. Vergnolle, C. Comera, J. Fioramonti, and L. Bueno, “Involvement of interleukin-1, prostaglandins and mast cells in rectal distension-induced colonic water secretion in rats,”, J. Hu, W.-Y. $6.50. Furthermore, immunofluorescence and toluidine blue staining revealed increased numbers of mast cells in the EAE group, and EAE increased histamine release from the colonic tissues. The Prunus persica fruit and its polyphenolic extract can also execute chemoprotective role against cancer through modulating in vivo xenobiotic metabolizing enzymes [17]. Taohong Siwu decoction (THSWD) originated from the traditional Chinese medicine book Yizong Jinjian of the Qing Dynasty, which is composed of Prunus persica … Unprocessed, blanched or stir-baked. It is described to have the function to treat disease like jaundice, hematemesis, hemorrhoids, amenorrhea, and tinea in classical Chinese medical documents and also used in nowadays Chinese medicine application. Super-Family. Rich Lady) inhibit tumor growth and metastasis of MDA-MB-435 breast cancer cells in vivo,”, M. Vizzotto, W. Porter, D. Byrne, and L. Cisneros-Zevallos, “Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells,”, J. Breza, O. Dzurny, A. Borowka et al., “Efficacy and acceptability of Tadenan® (Pygeum africanum extract) in the treatment of benign prostatic hyperplasia (BPH): A multicentre trial in central Europe,”, S. Larré, P. Camparo, E. Comperat et al., “Biological effect of human serum collected before and after oral intake of Pygeum africanum on various benign prostate cell cultures,”, J. Xia and L. Huagang, “Experimental study of anti-inflammation activity of root of prunus persica,”, Z. Jingquan, H. Deqiang, Z. Kunhe et al., “Experimental study of cytotoxicity on hepatocarcinoma with root of wild peach tree,”, N. Mao, R.-Z. And this cell cycle arresting effect can last for at least 2 days. Height Spread; 10m. This formula has been modified to create … After incubation for 30 min, the supernatant was frozen at −20°C for subsequent analysis of histamine in the supernatant. aganonuci persica (Schübler & Martens) Yü et Lu; Prunus persica var. To. Increasing concentrations of EAE (10−8, 10−7, 10−6, 10−5, 10−4, and 10−3 g/mL) were then added at 10 min intervals and the muscle contractions were recorded. Diels), Chuanxiong Rhizoma (Conioselinum anthriscoides 'Chuanxiong'), Persicae Seman (Prunus persica (L.) Batsch) and Carthami Flos (Carthamus tinctorius L.) at a weight ratio of 3: 4: 3: 2: 3: 2. The EAE-treated group was found to have many more mast cells in the colon compared with the control group; the number of mast cells/mm2 increased from to (, , Figure 3). (g) Statistical analysis of wound width of HepG2 cells with indicated treatment at indicated time point. It has been established that the enteric nervous system can regulate intestinal motility [16] and TTX is a useful pharmacological tool that can distinguish between neuronal and myogenic responses in isolated smooth muscle sections [17, 18]. (a) Omission of primary antibody. Recently, mast cells have been shown to be increased in constipated patients as a compensatory mechanism for impaired propulsive activity in these patients [38]. To test the effect of TSG on the growth of liver cancer cell HepG2, we treated the cells with TSG of different concentration and the cell viability was monitored at 24 and 48 hours. Harvest. Prunus persica var. 2015, Article ID 569853, 10 pages, 2015. https://doi.org/10.1155/2015/569853, 1Department of General Surgery, Hepato-Biliary-Pancreatic Institute, Lanzhou University Second Hospital, Lanzhou 730030, China, 2Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China, 3Disease Control and Prevention Center of Qingan, Tianshui 741600, China, 4Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Beijing 100053, China. Share. https://orcid.org. Flow cytometry (Canto II, BD bioscience, USA) was employed to analyze the cellular DNA content. Description : Seed of Prunus persicae: 1.2 - 1.8cm long. Mast cells in the EAE-treated group showed an increase in cell volume together with signs of degranulation compared with the control group. In line with the previous report in liver cancer cell SMMC7721 and SK-HEP-1, our data indicated that root of Prunus persica extract also induced a dose and time-dependent growth inhibition of HepG2 cells. Expected size. Order) 3 YRS . Pottosin I, Shabala S. 2016. It was introduced to Persia before Christian times and was spread by the Romans throughout Europe. But few studies have explored the pharmacological effect of Prunus persica (L.) Batsch flowers on gastrointestinal motility. Tryptase-immunoreactive mast cells were counted using a laser scanning confocal microscope (TCS SP5, Leica, Germany). [28] Sections of distal colon were equilibrated in Krebs solution with 95% O2 and 5% CO2 (1 mL) for 30 min; then EAE (10−6 g/mL) or the same volume of distilled water was added to the solution. The proposed electrochemical oscillation system was then used for the identification of P. persica, P. davidiana, and P. armeniaca. The specific name persica refers to its widespread cultivation in Persia, from where it was transplanted to Europe. The actual smell and taste. The rats were killed 1 week after administration of the last dose. Availability is subject to change. Prunus persica is commonly cultivated in West Asia, Europe, Himalayas and India up to an altitude of 1000 ft. [1], [20]. Prunus mume is a common fruit in Asia and used in traditional Chinese medicine. On the contrary, root of Prunus persica is a natural material and used as medical ingredient for hundreds of years in Chinese medicine. Everywhere in China. In the presence of atropine, the contractile effect of EAE was not blocked, indicating that cholinergic pathways are not responsible for the stimulant effect. According to the epidemiological data from the world health organization, Liver cancer is the second most lethal cancer type around the world. To further explore the effect of histamine release by mast cells involved in EAE-induced gastrointestinal motility, we measured histamine release in rat colonic tissues treated with EAE. Family. The regulation of gastrointestinal motility is a complex interplay between the enteric nervous system, gastrointestinal hormones, and immune cells. The enteric nervous system (ENS) regulates vital gut functions such as motility, epithelial transport, microcirculation, and barrier function [28]. It is remarkable that such common materials play so large a role in Chinese herb prescriptions for serious ailments. Accordingly, TSG treatment attenuated the expression level of the migration marker protein MMP3 and MMP9 as revealed by western blotting (Figure 3(h)). Mast cell analysis and measurement of histamine levels in rat colon tissues confirmed that the mechanism underlying the gastrointestinal activity involves release of histamine from mast cells. Norepinephrine (NE), a nonselective adrenergic agonist, was also used to explore the EAE-induced contraction. And, hepatocellular carcinoma (HCC) is the most common primary liver cancer [1]. After fixation in 70% ethanol overnight at −20°C, cells were rinsed again in PBS and stained in staining solution containing PI and RNase A. Ethanol extract of root of Prunus persica can inhibit in vitro HepG2 cells growth by inducing cell cycle arrest and migration suppression and in vivo tumor growth, suggesting potential application for novel anti-HCC drug development. The numbers of mast cells and the level of histamine were increased in the EAE group and the stimulatory effect of EAE was completely blocked by ketotifen. davidiana, carpophores of Poria cocos and root bark of Paeonia suffruticosa. peach; Variety. Hongchun Shen, Honglian Wang, Li Wang, Lu Wang, Menglian Zhu, Yao Ming, Sha Zhao, Junming Fan, En Yin Lai, "Ethanol Extract of Root of Prunus persica Inhibited the Growth of Liver Cancer Cell HepG2 by Inducing Cell Cycle Arrest and Migration Suppression", Evidence-Based Complementary and Alternative Medicine, vol. The contractile effect (amplitude %, frequency %) in the presence of EAE at a concentration of 10−6 g/mL was comparable to the basal level (Figures 1(b) and 1(c)). Find premium, high-resolution stock photography at Getty Images. (c) Detection of tryptase in EAE-treated group. Bitter, Sweet Leaf, Flower: Warm, dry. Prunus persica (L.) Batsch flowers have been used to treat indigestion and constipation in China since ancient times, but the mechanism of action of this herbal remedy is not fully understood. The dendrogram was divided into three main principal infrageneric … The peach tree, Prunus persica (L.) Batsch, which belongs to the family Rosaceae and the genus Amygdalus L., is widely cultivated in China and has been commonly used for centuries to treat different diseases. The species name resulted from an early misconception that the peach originated in Persia. All WHP single remedies are made to order; thus, the labels are printed on the same label stock as the orders are filled. The extract from each step was evaporated to dryness separately and redissolved in distilled water before use. To investigate the factors contributing to the inhibited growth of HepG2 cells, we checked the effect of TSG on cell cycle. We further investigated the mechanism underlying the action of EAE on muscle contractions. The OTC potency range of AMYGDALUS is 2x–30x, 1c–30c, 200c, 1m, 10m, 50m, and CM. The invention discloses a Chinese medicine for treating varicocele. Sin. Easy care. The crude methanolic extract and its various fractions namely petroleum ether, dichloromethane, chloroform and ethyl acetate were investigated for their antibacterial, antifungal, phytotoxic and insecticidal properties. Review articles are excluded from this waiver policy. Doses of 10−8, 10−7, 10−6, 10−5, 10−4, and 10−3 g/mL of EAE were then added separately; responses to EAE were demonstrated to be concentration-dependent. Adult male Sprague-Dawley rats (200–250 g) were provided by the Experimental Animal Center of the Xuanwu Hospital, Capital Medical University (Beijing, China). Despite a long history of the use of Prunus persica (L.) Batsch flowers in traditional Chinese medicine, few studies have explored the pharmacological effect of these preparations on gastrointestinal motility or the underlying mechanism of action. persica; Rosaceae, subgenus Amygdalus; synonym: Amygdalus persica) originated in China, but its wild ancestor, as well as where, when, and under what circumstances the peach was domesticated, is poorly known. In traditional Chinese medicine, Tao Ren is used to help invigorate the blood, remove stasis and moisten the intestines. In experience-based folk medicine, root of Prunus persica is also included in the prescription to ameliorate esophageal cancer [13]. The seeds, flowers, and leaves of P. persica have long been used as folk medicine in China and Korea [5–7]. Previously, the ethanol extract from the flowers of P. persica (Ku-35) was tested for protective effects against UV-induced damage in keratinocytes and fibroblasts in vitro. Best quality. It was said that the fruit had magical powers and it bestowed infinite life on whoever ate them. Furthermore, bark extract of Prunus africana, also named Pygeum africanum and a species showing evolutionarily close relationship with Prunus persica, has been successfully used in the treatment of benign prostatic hyperplasia [10, 11]. It is reinforced in combination with Hong Hua and combined with blood-activating and nourishing and menstruation-regulating herbs for treatment of amenorrhea due to blood stasis and dysmenorrhea. We conducted exon‐targeted resequencing of 129 genomes in the genus Prunus, mainly Japanese apricot ... (Prunus salicina), and peach (Prunus persica). The study has not been published yet. Since the 2nd day after cancer cell inoculation, the mice were intragastrically administrated with 4.3 mg/day of TSG or equivalent volume of solvent (10% ethanol) for 4 weeks. 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Arachidonate acid metabolites Address all correspondence to Hyun Pyo Kim preliminary pharmacological study indicated that TSG induced cell cycle One... Extracts from bark of Paeonia suffruticosa that TSG induced cell cycle analysis indicated that TSG caused sustained M/G2 phase..

prunus persica chinese medicine

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